This detailed volume provides methods to guide assay development, procedures designed to investigate the chemokine and glycosaminoglycan (GAG) networks, as well as their interactions, in a wide range of organs and tissues in disease and in health. The initial chapters in this book present in vivo models used to examine the roles of chemokines and GAGs in normal physiology and in the pathophysiology of disease. The book then explores present cell- and tissue-based in vitro assays to examine chemokine:GAG interactions. Finally, analytic approaches are presented that provide assays for measuring GAGs, chemokines, and cellular responses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chemokine-Glycosaminoglycan Interactions: Methods and Protocols serves as an ideal guide for researchers seeking to analyze chemokine and GAG functions, interactions, and molecular mechanisms in vivo and in vitro.

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